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. 2017 Feb 22;7:43161. doi: 10.1038/srep43161

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(A) About 10 minutes after in vitro fusion of the tobacco sperm cell with the central cell, wiskostatin was added to the medium. Note at the time of treatmentthe sperm nucleus was in transit to the polar nuclei. (Ai) DAPI fluorescence images of the same cell in A. (B) 15 minutes after being treated by wiskostatin, the tobacco sperm nucleus remained in the same location but its migration was stopped by wiskostatin treatment. (Bi) DAPI fluorescence images of the same cell in B. (C) Maize sperm nuclear remained at the entry site of the egg cell treated with wiskostatin. (Ci) DAPI fluorescence images of the same cell in C. Scale bar = 50 μm.