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. 2015 Aug 3;1(2):e000026. doi: 10.1099/mgen.0.000026

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© 2015 The Authors

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/).

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Fig. 1. — Phenotypic diversity of isolates during the persistent infection. (a) The colony size, antibiogram, vancomycin population analysis profile area under curve (VAN PAP/AUC) result and δ toxin activity of all 44 isolates are summarized. Increasing vancomycin resistance is indicated by colour (green is susceptible and red is resistant). Likewise, reduced colony size is indicated by red, whilst normal colony size is indicated by green. The sequential increase in antibiotic resistance is highlighted in red, with R indicating no growth and S indicating growth on the relevant antibiotic concentration. CIP, ciprofloxacin; RIF, rifampicin; VAN, vancomycin; TP, teicoplanin; FOX, cefoxitin; OX, oxicillin. (b) Analysis of δ-haemolysin production in 15, sequenced intermediate isolates demonstrated significant heterogeneity. Clinical isolate BPH1118 had no δ-haemolysin activity, whilst BPH1141 and BPH1141 had weak activity.