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. 2017 Feb 22;7:43382. doi: 10.1038/srep43382

Figure 1. The definition and characterisation of RNA-seq experimental materials in Rosa chinensis ‘Old Blush’.

Figure 1

(A) The four stages of petal development: FB_GP, green petals in the flower bud; FB_CP, colour-changing petals in the flower bud; FB_PP, pink petals in the flower bud; OF_PP, pink petals of the open flower. Plant materials grown under a 12 h light (6:00 am–18: 00 pm)/12 h dark (18:00 pm–6:00 am) photoperiod and a 25 °C day/18 °C night temperature regime in the greenhouse. Petals have been collected at 12:00 am. Scale bar = 5 mm. (B) The anthocyanin concentration in the petals of the four developmental stages. Cy-3,5-diglucoside (black line) is the main anthocyanin in petals of ‘Old Blush’. Cy-3-glucoside (blue line) is the other detectable anthocyanin. The inset images display a longitudinal cross-section of the petals at each stage of development. The error bars represent standard errors. (C) Petal protoplasts at stages FB_GP, FB_PP, and OF_PP and their respective mean protoplast diameter. Values represent the means and SD of three biological replicates. Asterisks denote significant differences compared with the diameter of stage FB_GP at P < 0.01 (Student’s t-test). (D) LC-MS chromatograms of Rosa chinensis ‘Old Blush’ petals at stage FB_PP. Absorbance was measured at 520 nm. Peak 1: Cy-3,5-diglucoside, m/z = 611.1; Peak 2: Cy-3-glucoside, m/z = 287.0 and 449.0.