Table 1. Concordance between NGMASTER results from draft genome assemblies using MEGAHIT and SPAdes, and previously published NG-MAST results.
MEGAHIT | SPAdes | Two-stage¶ | Total | |
---|---|---|---|---|
PRJEB2999* | 176 (95 %) | 184 (99 %) | 184 (99 %) | 186 |
PRJNA29335† | – | – | – | 1 |
PRJNA266539‡ | 162 (91 %) | 169 (94 %) | 178 (99 %) | 179 |
PRJNA298332§ | 199 (93 %) | 207 (97 %) | 208 (97 %) | 214 |
PRJEB14168|| | 50 (100 %) | 50 (100 %) | 50 (100 %) | 50 |
Total | 587 (93 %) | 610 (97 %) | 620 (98 %) | 630 |
§Closed reference genome NCCP11945 (Genbank accession CP001050.1) – in silico NG-MAST results reported by Demczuk et al. (2015).
||Local isolates with NG-MAST performed by PCR/Sanger sequencing.
¶Two-stage assembly: 1. NGMASTER run using rapid assembly with MEGAHIT; 2. NGMASTER also run using SPAdes if there was no result or a mixed result using MEGAHIT assembly.