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. 2016 Aug 25;2(8):e000082. doi: 10.1099/mgen.0.000082

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© 2016 The Authors

This is an open access article under the terms of the Creative Commons Attribution 4.04.0 International License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

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Fig. 1. — SDS–PAGE analysis of influenza B NS1 protein. (a). Time course infection of B/Lee/40 on HEK 293T cells. Cells were harvested at 4, 8, 12 and 16 h post infection and immunoblotted with αNS1. (b). HEK 293T cells were infected with B/Lee/40 virus (inf) or transfected with pCAGGS/NS1B-FLAG (Trans) and after 20 h the cell lysates were immunoblotted with αNS1. Mock Trans: Cells transfected with empty pCAGGS vector. Mock Inf: non-infected cells. (c). pCAGGS/NS1B-FLAG-transfected HEK 293T cell lysates were harvested at 4, 8, 12 and 16 h post transfection and immunoblotted using anti-FLAG. The full length (black arrow) and smaller (*) NS1 proteins are indicated.