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. 2016 Aug 25;2(8):e000082. doi: 10.1099/mgen.0.000082

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© 2016 The Authors

This is an open access article under the terms of the Creative Commons Attribution 4.04.0 International License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

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Fig. 5. — Mutational analysis of Influenza B NS1 protein. (a). Sequence alignment (between amino acids 81 and 102) of Lee-NS1 and the mutants Lee-NS1 D92N, Lee-NS1 D92A, Lee-NS1 M91A, Lee-NS1 P93A and Lee-NS1 S94P. Also shown are 117-NS1 and 117-NS1 P94S. (B–E). Protein expression profile of (b) Lee-NS1 wild-type (WT) and mutant Lee-NS1 D92A, (c). 132-NS1 wild-type (WT), 132-NS1 N92D, Lee-NS1 wild-type (WT) and Lee-NS1 D92N, (d). Lee-NS1 wild-type (WT), Lee-NS1 M91A, Lee-NS1 P93A, Lee-NS1 S94P and (e). 117-NS1 wild-type (WT) and 117-NS1 P94S. At 16 h post transfection, NS1 protein was detected by immunoblotting with anti-FLAG. pC: Cells transfected with empty pCAGGS vector. Protein bands corresponding to NS1-FLAG (black arrow) and p23-FLAG (*) are indicated.