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. 2017 Feb 15;37(7):1772–1784. doi: 10.1523/JNEUROSCI.2459-16.2017

Figure 3.

Figure 3.

TREM2 deletion in microglia leads to cell cycle arrest at the G1/S checkpoint. A, Trem2−/ or WT microglia were fixed, stained with DAPI, and analyzed by flow cytometry. B, Ratio of stage-specific (G1/G0, S, or G2/M) cell numbers to total cell numbers was quantified for comparison. The number of G1/G0 phase cells increased, whereas S-phase cells decreased in Trem2−/− microglia compared with WT microglia. C, Cyclin D1 and c-Myc mRNA levels were decreased in Trem2−/− microglia compared with WT cells as quantified by qRT-PCR. D, Cell lysates from Trem2−/− microglia were analyzed for Cyclin D1 and c-Myc by Western blotting. Protein levels were quantified by densitometry normalized to α-tubulin. Note that Cyclin D1 and c-Myc were downregulated in Trem2−/− microglia compared with WT cells. Data are plotted as mean ± SEM (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001.