Figure 6.

Effects of Malat1 silencing on major proinflammatory cytokines in mouse BMECs after OGD. A–C, Cultured BMECs were transfected with LNA-Malat1 GapmeR and scramble control for 48 h, and then subjected to 16 h OGD. The expression levels of E-selectin (A), MCP-1 (B), and IL-6 (C) were determined by qPCR and normalized to cyclophilin (n = 3). D, The protein level of E-selectin was determined by Western blotting, with β-actin as the loading control. Experiments were repeated three times, and representative blots are displayed. E, F, Protein levels of MCP-1 (E) and IL-6 (F) in Malat1 Ctrl and Malat1 GapmeR-treated BMEC culture medium were analyzed by ELISA (n = 3). Silencing of Malat1 by GapmeR treatment increased the expression of proinflammatory cytokines MCP-1, IL-6, and E-selectin in cultured BMECs after 16 h OGD. Data are mean ± SD. *p < 0.05 versus Malat1 Ctrl + OGD group. ^#p < 0.05 versus Malat1 Ctrl + Non-OGD group.