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. 2017 Feb 15;37(7):1900–1909. doi: 10.1523/JNEUROSCI.3222-16.2017

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Copyright © 2017 Akle, Stankiewicz et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 3. — Nighttime transition of dividing cells through G₂/M phases of the cell cycle in the cerebellum of adult zebrafish. A–C, Number of cells expressing pH3, a late G₂ and early M phase marker, and BrdU, a marker for S phase. A, Cells positive for pH3-only increase throughout the night, B, Cells with colocalized BrdU/pH3 label decline throughout the night. n = 3–6 fish per time point; mean ± SEM; *p < 0.05 relative to the trough in labeled cells (pH3-only, ZT11; BrdU/pH3, ZT23). The gray background indicates night, 14:10 LD cycle. Fish were collected at specified ZT, after a 2 h in vivo exposure to BrdU. ZT14 is lights-off time. C, Representative confocal image: cells labeled for pH3-only (red), BrdU-only (green), and BrdU/pH3 merge (yellow). Scale bar, 20 μm.