Fig. 2.

IRE1α-XBP1 pathway regulates interleukin-6 expression in melanocytes and melanoma cells. a XBP1 splicing levels in melanocytes (HEMn-MP and HEMn-DP) and melanoma cells (Mel-RMu, MM200, Mel-CV, IgR3, A2058 and SkMel-28). mRNA levels of XBP1s and IL-6 as determined by real-time PCR in HEMn-MP (b) and Mel-RMu (c) cells transfected with plasmids pCMV-XBP1s (XBP1s) or pCMV (ctrl.). d The IRE1α protein levels were determined by immunoblotting in both HEMn-MP and Mel-RMu cells transfected with plasmids pCMV-IRE1α (IRE1) or pCMV (ctrl.). XBP1 splicing (e) and IL-6 (f) expression levels were determined by real-time PCR in IRE1α-overexpressing HEMn-MP and Mel-RMu cells with 4μ8C treatment. The results are from at least three independent experiments. The data are presented as the mean ± s.e.m. *P < 0.05, **P < 0.01 by Student’s t test or two-way ANOVA