Fig. 1.

Maximum-parsimony phylogeny of the drum-skin-associated isolates. Reconstructed in mega6 (Tamura et al., 2013) using the spr algorithm, with 572 SNPs identified by WGS of the 24 isolates using Ames Ancestor as reference. Bootstrap values (1000 replicates) are shown, but likely underestimate confidence in clonal populations with little homoplasy (Pearson et al., 2009). Branch length in units of number of changes over the whole sequence. There are no homoplasies in the topology. Location and sample source are shown for each isolate, MLVA-8 profiles are indicated in parentheses.