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. 2016 Nov 30;2(11):e000095. doi: 10.1099/mgen.0.000095

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© 2016 The Authors

This is an open access article under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

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Fig. 5. — LPS O-antigen biosynthesis in O1b and O2 strains of Y. ruckeri. (a) Gene order (trailing strand) in reference genome of QMA0440 (O1b) and QMA0436 (O2). Pale grey shows genes encoding polysaccharide-modifying enzymes derived from the QMA0440 genome annotation. The flippase and polymerase are shown in dark grey and adjacent tRNA in green. (b) SDS-PAGE gel (12 % acrylamide) showing purified LPS from O1b isolates QMA0401 (1), QMA0404 (2), QMA0424 (3), QMA0432 (4) and QMA0440 (7) and O2 isolates QMA0436 (5) and QMA0438 (6). Gels were stained with silver after initial oxidation with sodium periodate according to the method of Tsai & Frasch (1982).