Table 1.

Patient demographic information and phenotypic and genotypic characteristics of four invasive capsule null Neisseria meningitidis isolates identified through national laboratory-based surveillance in South Africa, 2003–2013

Characteristic
Patient	1	2	3 (Episode 1)a	3 (Episode 2)a
Gender	Male	Male	Male	Male
Age category (years)	45–64	15–24	5–9	5–9
HIV status	Negative	Unknown	Negative	Negative
Antiretroviral use	Not applicable	Unknown	Not applicable	Not applicable
Underlying disease	Diabetes mellitus, COPD	Unknown	C6 deficiency	C6 deficiency
Year of disease presentation	2006	2010	2011	2012
Province	Western Cape	Gauteng	Free State	Free State
Patient outcome	Recovered	Recovered	Recovered	Recovered
Specimen type	Pleural aspirate	CSF	CSF	Blood
Isolate
Minimum Inhibitory Concentrations (μg/ml)
Penicillin G	0.032 (S)	0.064 (S)	0.064 (S)	0.047 (S)
Ceftriaxone	≤0.002 (S)	≤0.002 (S)	≤0.002 (S)	≤0.002 (S)
Trimethoprim-sulfamethoxazole	8 (R)	12 (R)	3 (R)	3.8 (R)
Chloramphenicol	0.75 (S)	1 (S)	1 (S)	0.38 (S)
Rifampicin	0.008 (S)	0.032 (S)	0.064 (S)	0.032 (S)
Ciprofloxacin	0.008 (S)	0.008 (S)	0.006 (S)	0.006 (S)
Molecular characterization
cnl allele (NEIS2743)	12	2	2	2
Strain designation	NG: P1.7-2,30: F1-2: ST-53 (cc53)	NG: P1.18-11, 42-2: FΔ: ST-192 (cc192)	NG: P1.18-11, 42-2: FΔ: ST-192 (cc192)	NG: P1.18-11, 42-2: FΔ: ST-192 (cc192)
Genome information
Approx genome size (bp)	2,104,685	2,040,849	1,995,940	2,003,633
No. contigs	119	111	447	489
Neisseria PubMLST.org identification number	29312	29306	41961	41860

Abbreviations: COPD chronic obstructive pulmonary disease, C6 sixth complement component, CSF cerebrospinal fluid, S susceptible, R resistant, NG non-groupable, P1 PorA, F FetA, ST sequence type, cc clonal complex, Δ gene deletion

^aPatient three presented with two episodes of invasive meningococcal disease in 2011 (episode 1) and 2012 (episode 2, 8 months later), respectively. He was diagnosed with deficiency of the sixth complement component (C6)