Table 4.
Nucleotide and amino acid sequence identity between BtHBVRs3364 and representative orthohepadnavirus strainsa
| Hepadnavirus (no. of strains compared) | Degree of identity (%) | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Full genome | S gene (1–672) | X gene (1217–1645) | C gene (1657–2301) | P gene (2147–1475) | |||||
| Nucleotides | Nucleotides | Amino acids | Nucleotides | Amino acids | Nucleotides | Amino acids | Nucleotides | Amino acids | |
| Asian roundleaf bat hepadnavirus (3) | 81.2–81.9 | 90.5–93.9 | 83.5–90.2 | 86.7 | 75.4 | 85.2–85.3 | 91.2–91.7 | 80.1–80.9 | 77.4–77.9 |
| African horseshoe bat hepadnavirus (1) | 72.2 | 89.0 | 84.8 | 78.8 | 65.5 | 75.2 | 77.9 | 71.8 | 65.9 |
| African roundleaf bat hepadnavirus (4) | 72.0–72.1 | 89.0–89.2 | 86.2 | 81.8–82.1 | 70.4–71.1 | 76.9–77.1 | 77.9 | 72.3–72.4 | 65.6–66.0 |
| Long-fingered bat hepadnavirus (3) | 69.0–69.2 | 79.7–80.1 | 67.4 | 69.2–70.2 | 49.3–51.4 | 74.2–74.5 | 75.6–76.0 | 68.1–68.3 | 61.5–61.8 |
| Tent-making bat hepadnavirus (4) | 56.7–56.8 | 73.5–74.2 | 61.0–61.9 | 58.9–59.3 | 43.4–44.1 | 51.6 | 49.5 | 56.8–57.1 | 49.1–49.2 |
| Primate HBV (15) | 59.5–61.1 | 76.5–77.8 | 65.0–66.8 | 62.1–64.9 | 46.5–52.1 | 61.0–67.9 | 63.0–66.5 | 57.0–59.7 | 50.2–52.0 |
| Woolly monkey hepadnavirus (1) | 61.5 | 75.3 | 67.1 | 63.2 | 50.0 | 64.2 | 61.4 | 60.3 | 51.9 |
| Ground squirrel hepadnavirus (2) | 64.0–65.1 | 78.7–78.8 | 67.9–68.3 | 60.6–63.9 | 45.1–46.5 | 71.1–71.4 | 70.9–74.0 | 58.9–59.1 | 49.3–50.2 |
| Woodchuck hepadnavirus (1) | 59.8 | 77.9 | 67.4 | 63.9 | 47.2 | 69.8 | 72.5 | 58.8 | 50.9 |
| Duck hepadnavirus (1) | 31.1 | 44.1 | 28.7 | NA | NA | 31.3 | 20.9 | 35.1 | 23.2 |
aThe sequences were aligned using MAFFT. The evolutionary analyses were conducted using MEGA version 7. The GenBank accession numbers are as follows: KF939648, KF939649, and KF939650 for the Asian roundleaf bat hepadnaviruses; KC790377 for the African horseshoe bat hepadnavirus; KC790374, KC790375, KC790373, and KC790376 for the African roundleaf bat hepadnaviruses; KC790378, KC790379, KC790380, and KC790381 for the Tent-making bat hepadnaviruses; AF160501, AY090454, X69798, AF193863, U46935, AB486012, AF305327, AB117758, D23678, AM282986, AF241409, AB205192, FJ349213, AB126581, and JN040752 for the Primate HBVs; AF046996 for the woolly monkey hepadnavirus; NC 001484 and U29144 for the ground squirrel hepadnaviruses; NC_004107 for the woodchuck hepadnavirus; and EU429324 for the duck hepadnaviruses. NA not available