Table 5.

Nucleotide and amino acid sequence identity between BtHEVMd2350 and representative hepevirus strains^a

Hepevirus (no. of strains compared)	Degree of identity (%)
	Full genome	ORF1 (genome positions 56–4699)		ORF2 (genome positions 4700–6607)		ORF3 (genome positions 4779–5192)
	Nucleotides	Nucleotides	Amino acids	Nucleotides	Amino acids	Nucleotides	Amino acids
Bat hepevirus (1)	67.8	65.5	72.0	70.9	79.2	73.2	44.3
Avian hepevirus (4)	50.9–51.7	48.2–49.0	44.3–44.5	46.0–47.3	44.4–44.9	19.9–21.1	5.3–6.1
HEV genotype 3 (22)	44.6–46.0	43.4–45.5	39.1–40.9	47.4–49.9	44.6–47.4	30.7–31.8	14.8–18.9
HEV genotype 4 (5)	45.2–46.0	44.9–45.9	40.4–41.0	47.5–48.2	45.8–47.1	30.0–31.6	14.6–16.3
HEV genotype 1 (3)	45.6–45.9	45.0–45.5	40.5–41.1	48.7–49.3	46.6–46.9	31.9–32.6	17.1
HEV genotype 2 (1)	45.6	45.3	40.8	49.7	47.1	31.6	17.1
Rodent hepevirus (3)	44.0–44.4	45.9–46.1	41.6–42.0	47.9–48.4	45.4–46.7	25.2–27.4	9.0
Ferret hepevirus (1)	44.0	46.4	42.6	48.3	46.0	25.5	8.0–12.8
Trout hepevirus (1)	33.7	34.9	24.5	30.2	15.5	21.1	9.8

^aThe sequences were aligned using MAFFT. The evolutionary analyses were conducted using MEGA version 7. The GenBank accession numbers are as follows: JQ001749 for the bat hepevirus; AM943646, AM943647, KF511797, and AY535004 for the avian hepeviruses; AB189070, AB189075, AP003430, AB091394, AB222183, AB073912, AY115488, AF060669, AF082843, HQ389544, JN564006, AB290312, FJ998008, FJ705359, KC618402, AF455784, AB248521, EU360977, EU495148, FJ956757, FJ906895, and JQ013793 for the HEVs genotype 3; AB856243, AB602440, AB161717, AJ272108, and EU366959 for the HEVs genotype 4; AY230202, AF076239, and M80581 for the HEVs genotype 1; M74506 for HEV genotype 2; GU345042, AB847306, and JX120573 for the rodent hepeviruses; JN998607 for the ferret hepevirus; and NC_015521 for the trout hepevirus