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. 2017 Feb 21;48:12. doi: 10.1186/s13567-017-0416-7

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Replication of CEV in gill explant cultures and in primary fin cultures of various carp strains. Cultures were obtained from koi (Koi) and common carp from following strains: Amur wild carp (AS), Ropsha (Rop) Prerov (PS). Cultures were infected with CEV from the carp strain for 48 h at 15 or 25 °C. The CEV was re-isolated from KSD affected carp. As a surrogate for virus replication, the expression of mRNA encoding the viral core protein P4a (CEV P4a mRNA levels) is shown as mean (+SD) copy number of the P4a gene normalised against 100 000 copies of the carp 40S ribosomal protein S11 from n = 3 cultures.