Table 2.
CEV load and replication in tissues from carp after experimental cohabitation with KSD affected common carp (Experiment no. Co I)
| Koi | PS | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Day 2 | Day 4 | Day 6 | Day 10 | Day 15 | Day 2 | Day 4 | Day 6 | Day 10 | Day 15 | |
| A (DNA) | ||||||||||
| Gill | ||||||||||
| Mean | 1.72E+04 | 6.84E+04 | 2.10E+05 | 3.35E+05 | 1.50E+05 | 3.41E+03 | 3.95E+02 | 2.88E+05 | 3.71E+05 | 7.21E+05 |
| Median | 1.92E+04 | 3.19E+04 | 1.06E+04 | 2.28E+04 | 1.53E+05 | 3.39E+03 | 1.00E+02 | 1.10E+03 | 9.03E+03 | 2.30E+05 |
| SD | 1.10E+04 | 9.71E+04 | 3.15E+05 | 7.13E+05 | 1.34E+05 | 2.25E+03 | 6.26E+02 | 6.42E+05 | 6.85E+05 | 1.21E+06 |
| Skin | ||||||||||
| Mean | 1.01E+02 | 4.90E+01* | 3.72E+02 | 1.01E+02 | 1.13E+04 | 1.84E+01 | 9.20E+00* | 2.72E+00 | 7.52E+01 | 1.44E+02 |
| Median | 2.90E+01 | 2.39E+01* | 1.22E+02 | 8.61E+00 | 1.42E+03 | 6.13E+00 | 1.00E+00* | 1.03E+00 | 7.13E+01 | 6.78E+01 |
| SD | 1.65E+02 | 7.67E+01* | 5.42E+02 | 2.08E+02 | 2.21E+04 | 2.70E+01 | 1.83E+01* | 4.42E+00 | 5.65E+01 | 1.69E+02 |
| Head_Kidney | ||||||||||
| Mean | 4.81E+01 | 3.54E+01 | 1.99E+01 | 5.10E+02 | 2.94E+04 | 3.93E+00 | 9.06E+00 | 1.34E+01 | 1.52E+02 | 1.67E+02 |
| Median | 2.65E+00 | 1.14E+01 | 4.66E+00 | 2.05E+01 | 4.78E+02 | 0.00E+00 | 0.00E+00 | 2.61E+00 | 1.00E+00 | 0.00E+00 |
| SD | 7.38E+01 | 5.60E+01 | 3.78E+01 | 7.99E+02 | 6.44E+04 | 6.21E+00 | 2.03E+01 | 2.62E+01 | 3.16E+02 | 3.17E+02 |
| Kidney | ||||||||||
| Mean | 0.00E+00 | 2.02E+01 | 8.30E+00 | 1.72E+00 | 1.38E+02 | 0.00E+00 | 0.00E+00 | 0.00E+00 | 5.35E+01 | 1.32E+02 |
| Median | 0.00E+00 | 0.00E+00 | 2.98E+00 | 0.00E+00 | 7.82E+00 | 0.00E+00 | 0.00E+00 | 0.00E+00 | 2.16E+01 | 1.06E+01 |
| SD | 0.00E+00 | 4.27E+01 | 1.51E+01 | 3.84E+00 | 2.86E+02 | 0.00E+00 | 0.00E+00 | 0.00E+00 | 7.90E+01 | 2.79E+02 |
| B (RNA) | ||||||||||
| Gill | ||||||||||
| Mean | 2.46E+02 | 4.15E+03 | 8.20E+03 | 8.74E+03 | 2.03E+03 | 1.01E+02 | 1.64E+01 | 3.11E+03 | 5.60E+03 | 1.63E+04 |
| Median | 1.52E+02 | 5.01E+02 | 6.96E+01 | 7.45E+02 | 2.31E+03 | 6.48E+01 | 7.84E+00 | 2.89E+00 | 5.20E+02 | 1.43E+04 |
| SD | 2.12E+02 | 7.65E+03 | 1.19E+04 | 1.84E+04 | 1.23E+03 | 1.00E+02 | 2.35E+01 | 6.94E+03 | 1.14E+04 | 1.91E+04 |
| Skin | ||||||||||
| Mean | 0.00E+00 | 0.00E+00 | 1.89E+00 | 8.29E−01 | 0.00E+00 | 0.00E+00 | 2.27E−01 | 0.00E+00 | 0.00E+00 | 1.06E+00 |
| Median | 0.00E+00 | 0.00E+00 | 0.00E+00 | 0.00E+00 | 0.00E+00 | 0.00E+00 | 0.00E+00 | 0.00E+00 | 0.00E+00 | 0.00E+00 |
| SD | 0.00E+00 | 0.00E+00 | 2.84E+00 | 1.85E+00 | 0.00E+00 | 0.00E+00 | 5.07E−01 | 0.00E+00 | 0.00E+00 | 2.36E+00 |
| Head Kidney | ||||||||||
| Mean | 2.00E−01 | 2.47E−01 | 2.00E−01 | 2.90E−01 | 2.20E+03 | 8.43E−01 | 0.00E+00 | 0.00E+00 | 9.69E−01 | 1.03E+00 |
| Median | 0.00E+00 | 0.00E+00 | 0.00E+00 | 0.00E+00 | 6.94E+00 | 0.00E+00 | 0.00E+00 | 0.00E+00 | 0.00E+00 | 0.00E+00 |
| SD | 4.47E−01 | 5.51E−01 | 4.47E−01 | 6.48E−01 | 4.90E+03 | 1.40E+00 | 0.00E+00 | 0.00E+00 | 1.66E+00 | 1.80E+00 |
| Kidney | ||||||||||
| Mean | 4.92E+00 | 0.00E+00 | 2.09E+00 | 2.00E−01 | 1.36E+02 | 0.00E+00 | 0.00E+00 | 2.00E−01 | 0.00E+00 | 1.82E+02 |
| Median | 1.16E+00 | 0.00E+00 | 0.00E+00 | 0.00E+00 | 3.67E+01 | 0.00E+00 | 0.00E+00 | 0.00E+00 | 0.00E+00 | 2.01E+00 |
| SD | 7.50E+00 | 0.00E+00 | 4.15E+00 | 4.47E−01 | 2.61E+02 | 0.00E+00 | 0.00E+00 | 4.47E−01 | 0.00E+00 | 3.80E+02 |
Carp edema virus load was measured by qPCR as copy numbers of virus specific DNA and, as a surrogate for virus replication, the expression of the mRNA encoding the gene of the CEV core protein P4a (CEV P4a) was analysed in the gills, skin, kidney, and head kidney of koi and Prerov (PS) common carp during cohabitation with carp affected by KSD. Samples were collected 2, 4, 6, 10, 15 days post-exposure from n = 5 fish per day. The data on virus load (A) and expression of viral mRNA (B) are shown as mean, median and standard deviation (SD) of genome copies in 250 ng of isolated DNA from n = 4 fish (CEV load) or of the copy numbers of mRNA encoding the P4a gene normalised against 100 000 copies of the carp 40S ribosomal protein S11 (CEV replication). Symbol “*”indicate significant differences at p ≤ 0.05 between carp strains.