Table 3.
mRNA expression of genes involved in the type I interferon response of common carp
| Carp strain | CEV genogroup I | CEV genogroup IIa | ||||
|---|---|---|---|---|---|---|
| Control | Day 6 | Day 11 | Control | Day 6 | Day 11 | |
| Cyca IFN a2 | ||||||
| Koi | ||||||
| Mean | 6.1E+01 a | 1.9E+02 a | 1.4E+02 a | 6.1E+01 a | 1.8E+02 a | 8.4E+02 b* |
| SD | 3.3E+01 | 1.2E+02 | 9.5E+01 | 3.3E+01 | 1.0E+02 | 1.9E+02 |
| PS | ||||||
| Mean | 6.6E+01 a | 1.5E+02 a | 1.2E+02 a | 6.6E+01 a | 1.2E+02 a | 9.5E+01 a |
| SD | 7.5E+00 | 2.4E+01 | 7.1E+01 | 7.5E+00 | 4.9E+01 | 3.3E+01 |
| Rop | ||||||
| Mean | 7.9E+01 a | 1.3E+02 ab | 9.6E+01 ab | 7.9E+01 a | 9.3E+01 a | 8.5E+01 a |
| SD | 4.1E+01 | 4.3E+01 | 4.2E+01 | 4.1E+01 | 4.0E+01 | 4.4E+01 |
| AS | ||||||
| Mean | 1.6E+02 a | 4.8E+01 b* | 3.8E+01 b* | 1.6E+02 a | 1.2E+02 a | 1.2E+02 a |
| SD | 1.2E+02 | 1.9E+01 | 1.8E+01 | 1.2E+02 | 4.7E+01 | 4.2E+01 |
| Cyca Viperin | ||||||
| Koi | ||||||
| Mean | 7.3E+02 a | 4.2E+04 a* | 3.6E+04 a* | 7.3E+02 a | 3.1E+04 a* | 2.5E+04 a* |
| SD | 3.2E+02 | 1.5E+04 | 2.1E+04 | 3.2E+02 | 1.0E+04 | 5.3E+03 |
| PS | ||||||
| Mean | 9.0E+02 a | 3.8E+04 a* | 2.7E+04 ab* | 9.0E+02 a | 9.4E+03 b* | 3.6E+03 b* |
| SD | 2.6E+02 | 2.6E+03 | 1.3E+04 | 2.6E+02 | 4.4E+03 | 1.2E+03 |
| Rop | ||||||
| Mean | 9.0E+02 a | 2.3E+04 a* | 1.2E+04 ab* | 9.0E+02 a | 1.5E+04 ab* | 4.0E+03 b* |
| SD | 6.7E+02 | 1.8E+04 | 1.0E+04 | 6.7E+02 | 1.9E+03 | 2.8E+03 |
| AS | ||||||
| Mean | 2.2E+03 a | 6.3E+03 b* | 7.7E+03 b* | 2.2E+03 a | 7.0E+03 b* | 1.4E+03 b* |
| SD | 2.9E+03 | 5.2E+03 | 4.0E+03 | 2.9E+03 | 1.6E+03 | 2.4E+02 |
| Cyca PKR | ||||||
| Koi | ||||||
| Mean | 3.6E+02 a | 5.6E+03 ab* | 4.5E+03 ab* | 3.6E+02 a | 5.4E+03 a* | 4.8E+03 a* |
| SD | 8.4E+01 | 9.8E+02 | 2.2E+03 | 8.4E+01 | 1.6E+03 | 1.1E+03 |
| PS | ||||||
| Mean | 7.3E+02 a | 7.5E+03 a* | 4.9E+03 a* | 7.3E+02 a | 3.1E+03 ab* | 1.1E+03 bc* |
| SD | 1.2E+02 | 7.6E+02 | 5.0E+02 | 1.2E+02 | 1.6E+03 | 2.1E+02 |
| Rop | ||||||
| Mean | 9.3E+02 a | 3.6E+03 bc* | 2.6E+03 ab* | 9.3E+02 a | 4.7E+03 a* | 1.7E+03 b* |
| SD | 2.2E+02 | 1.3E+03 | 1.2E+03 | 2.2E+02 | 9.4E+02 | 1.0E+03 |
| AS | ||||||
| Mean | 7.4E+02 a | 2.0E+03 c* | 1.9E+03 b* | 7.4E+02 a | 2.0E+03 b* | 6.5E+02 c* |
| SD | 5.8E+02 | 1.0E+03 | 7.2E+02 | 5.8E+02 | 5.0E+02 | 1.1E+02 |
Expression levels of genes encoding for common carp Cyprinus carpio (Cyca) virus-induced interferon a2 (Cyca IFN a2), the interferon induced proteins viperin (Cyca Viperin), and RNA dependent protein kinase (Cyca PKR) were measured in the gills during infection with carp edema virus from the genogroup I or genogroup IIa. Expression levels are shown as means and standard deviations (SD) of the copy number of mRNA encoding the gene normalised against 100 000 copies of the common carp 40S ribosomal protein S11 from n = 4 fish. Symbol “*” indicates significant differences at p ≤ 0.05 between infected and control individuals. Different letters indicate significant differences at p ≤ 0.05 between carp strains.