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. 2017 Feb 22;7:43251. doi: 10.1038/srep43251

Figure 6. Intermittent fasting restores autophagy in DOX-induced cardiotoxicity.

Figure 6

(a) Representative images of LC3 immunohistochemistry in LVs on heart sections from fed or fasted WT and UVRAG-deficient mice 5 days after acute DOX or vehicle treatment. Scale bar: 40 μm. (b) Quantification of LC3-positive dots in LVs in the experiments as illustrated in (a). n = 3 mice for each group. (c) Representative images of LC3 immunohistochemistry in LVs on heart sections from fasted WT and UVRAG-deficient mice at 4 weeks of DOX treatment in chronic cardiotoxicity. Scale bar: 40 μm. (d) Quantification of LC3-positive dots in LVs in the experiments as illustrated in (c). n = 3 mice for each group. (e) Western blot detection of LC3 and p62 in LVs from fed or fasted WT and UVRAG-deficient mice 5 days after acute DOX or vehicle treatment. (f) Quantification of LC3 II protein abundance in the experiments as illustrated in (e). n = 3 mice for each group. (g) Quantification of p62 protein abundance in the experiments as illustrated in (e). n = 3 mice for each group. (h) Western blot analysis of LC3 II and p62 in LVs from fed or fasted WT and UVRAG-deficient mice at 4 weeks of DOX treatment in chronic cardiotoxicity. (i) Quantification of LC3 II protein abundance in the experiments as illustrated in (h). n = 3 mice for each group. (j) Quantification of p62 protein abundance in the experiments as illustrated in (h). n = 3 mice for each gorup. *P < 0.05 vs. WT + DOX + Fed, #P < 0.05 vs. UVRAG−/− + DOX + Fed, §P < 0.05 vs. WT + DOX + Fasted.