The proteins were oriented by superimposing the CD59D22A molecule of the VLYml-CD59D22A complex onto the CD59D22A of the ILYml-CD59D22A complex. Interfacial residues mentioned in the text are shown with key residues labeled, with those from CD59D22A in italics. The UDP loop is labeled with the Thr-Leu cholesterol-recognition motif (CRM) residues on L1 indicated.
(A–C) VLYml (blue) and CD59D22A (gray).
(D–F) ILYml (green) and CD59D22A (orange).
Close-up views are shown of the CD59D22A-CDC interfaces for VLYml (B and C) and ILYml (E and F) with the Y-X-Y-X14-R-S CD59-motif residues and their contact residues in CD59D22A labeled.
See also Figures S2, S5, and S6.