The NPR1 protein (1,061 amino acids) is comprised of three domains: extracellular domain, kinase homology domain, and guanylate cyclase domain. The effects of the 14 low-frequency and rare variants after adjustment for age, age2, sex, and body mass index on mean arterial pressure are shown for higher (tan) or lower (purple) values in mm Hg; dot area is proportional to the number of minor allele carriers. The minor allele of rs35479618 (MAF ∼ 0.012, E967K), was carried by 3,164 participants. The minor allele of rs201787421 (MAF ∼ 2.6×10−5 R782Q), was carried by 5 participants.