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. 2017 Jan 30;114(7):1536–1541. doi: 10.1073/pnas.1620312114

Fig. 3.

Fig. 3.

Dependence of chromatin transcription on protein factors and acetyl-CoA. (A) Repressed PHO5 chromatin was transcribed as in Fig. 1C with transcription proteins at 1×, 0.8×, and 0.57× concentrations, except for the omission of SAGA, SWI/SNF, Mediator, and TFIIS as indicated. Transcription levels were normalized to the value obtained from the complete reaction (percentage). (B) As in A with acetyl-CoA at the concentrations indicated. The ratios of transcription levels with and without acetyl-CoA are plotted on the ordinate. (C) Effect of gcn5 bromoΔ SAGA on chromatin transcription. Repressed (pho4Δ) PHO5 chromatin was transcribed as in Fig. 1C with transcription proteins at 1× concentration, except that gcn5 bromoΔ SAGA was substituted for WT SAGA where indicated. Transcription levels were normalized to the value obtained from the reaction with WT SAGA (percentage).