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SDS/PAGE of proteins for in vitro transcription. Coomassie staining of each factor for in vitro transcription subjected to SDS/PAGE. The labels on the right side denote the factor or its subunits. Mutant SAGA complexes were purified from the sgf29Δ, gcn5 bromoΔ-13Myc, and spt3Δ yeast strains (Table S2). SDS/PAGE and MS (Table S1) confirmed that the mutant SAGA complexes contained all of the subunits, except for the deleted subunit, as shown previously (22, 25, 37).
