Fig. 2.

APC ablation results in SFK-dependent YAP activation in intestinal organoids. (A) WT and APC-null SI organoids were lysed and analyzed for expression and phosphorylation of the indicated proteins by immunoblotting (IB). (B) RNAs from WT and APC-null enteroids and indicated transcripts were analyzed by quantitative RT-PCR (qRT-PCR). Results are means ± SEM (n = 3). *P < 0.05. (C) WT and APC-null enteroids were treated with the indicated inhibitors (PP2, AZD0530, and DBZ at 10 μM, and AZD1480 and Ruxolitinib at 3 μM) or vehicle (DMSO) for 24 h. Total lysates were IB-analyzed with the indicated antibodies.