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. 2017 Jan 27;114(7):1643–1648. doi: 10.1073/pnas.1620290114

Fig. 3.

Fig. 3.

APC ablation up-regulates gp130, IL-6R, and IL-11R expression. (A) WT and APC-null SI enteroids were analyzed for expression of the indicated mRNAs by qRT-PCR. Results are means ± SEM (n = 3). *P < 0.05. (B) WT and APC-null SI enteroids were lysed and IB-analyzed for the indicated proteins. (C) WT and Apc−/− SI enteroids were stimulated with IL-6 (100 ng/mL), IL-11 (100 ng/mL), or IL-6 (100 ng/mL) + sIL-6R (100 ng/mL) for 30 min, lysed, and IB-analyzed for protein expression and phosphorylation. (D) WT and APC-deficient human colon organoids were lysed and IB-analyzed for the indicated proteins. (E) Paraffin-embedded sections of surgically removed human CRC (n = 17) and matched normal colon sections (n = 7) were stained with a gp130 antibody; 65% of the cancer samples (11 of 17) showed similar gp130 positivity to the three examples depicted here. (F) Paraffin-embedded colon sections from tumor-bearing CPC-APC mice were stained with a gp130 antibody. (Scale bars, 100 μm.)