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. 2017 Feb 1;114(7):1678–1683. doi: 10.1073/pnas.1610223114

Fig. S2.

Fig. S2.

Additional controls for Src rescue of Taz from β-TrCP degradation. (A) HEK293 cells were transfected with Taz, β-TrCP2, and active Src, as indicated. MG-132, an inhibitor of proteasomal degradation, was added 24 h after transfection, at 25 μM, for 4 h. Cells were harvested and analyzed by Western blot, using the indicated antibodies. (B) mRNA levels for TrCP1 and TrCP2 in siTrCP1/2 transfected cells (n = 3). (C) HEK293 cells were transfected with Taz, β-TrCP2, and PyMT, as indicated, and then subjected to Western blotting, using indicated antibodies. *P < 0.05.