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NbCDPKiso2 is subgroup II CDPK. The full-length amino acid sequences of CDPKs from Arabidopsis (At), tobacco (Nt), potato (St), tomato (Sl), and N. benthamiana (Nb) were aligned and analyzed with ClustalW and MEGA7 algorithms. The CDPK family is divided into four subgroups (I–IV). The branched lengths are proportional to divergence and the scale of 0.05 indicates 5% change.
