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. 2017 Jan 30;114(7):E1062–E1071. doi: 10.1073/pnas.1617309114

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Fig. 3. — Sequence elements in the URA3 promoter are necessary and sufficient for cohesion. (A) The poly(dA:dT) tract is necessary for cohesion. Strains MSB7 (URA3f), HJ5 (orf_trunc), HJ6 (tata_trunc), HJ7 (uas_trunc), HJ19 (da:dt_trunc), HJ4 (full_trunc), and HJ24 (Δuf) were arrested in M phase by Cdc20 depletion. Strains HJ6388 (Δda:dt) and JSC97 (URA3r) were arrested with nocodazole. (B) DNA sequence surrounding the poly(dA:dT) element in the URA3 promoter. Underlines highlight the portions of the element that were deleted in the Δda:dt and Δuf constructs. (C) The poly(dA:dT) and UF elements generate cohesion in the lys2 context. Strains CSW91 (lys2 cassette alone), HJ22 (URA3 promoter add back), HJ23 (UF-dA:dT add back), and MSB155 (dA:dT alone add back) were arrested in M phase with nocodazole.