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. 2017 Jan 30;114(7):E1062–E1071. doi: 10.1073/pnas.1617309114

Fig. 6.

Fig. 6.

DNA circularization or an opposing transcribed gene prevents transcription-dependent loss of URA3 cohesion. (A) Experimental flowchart. Media containing uracil was replaced with media lacking uracil to induce URA3 transcription at the various times indicated during cell growth for the cohesion assay. (B) DNA circularization before URA3 induction prevents cohesion loss. Strain MSB35 (URA3f-git1Δ) was used. (C) A convergently oriented GIT1 gene prevents cohesion loss if URA3 transcription is induced after M phase. Strain MSB7 (URA3f) was used. (D) Orientation dependence of the SpHIS5MX gene in preventing transcription-dependent loss of URA3 cohesion. Strains MSB30 (URA3f-SpHIS5MXf) and MSB31 (URA3f-SpHIS5MXr) were used.