Table S1.
Yeast strains
| Name | Genotype | Progenitor | Ref. |
| W303-1A | MATa ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 | 54 | |
| PJ1 | MATa ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 | W303-1A | 55 |
| RDY213 | MATa hmlΔ::(loxP-kanMX-loxP)2 hmrΔ::RS-HMR-tT(AGU)C-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 scc1-73 can1-100 his3-11,15 trp1-1 ura3-1 bar1Δ::hisG | 28 | |
| RDY215 | MATa hmrΔ::RS-HMR-tT(AGU)C-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3-1 | 28 | |
| CSW91 | MATα lys2Δ::RS-lys2-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 can1-100 his3-11,15 trp1-1 ura3-1 | 56 | |
| K5824 | MATa smc3-42 ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 psi+ | 1 | |
| SG179.4 | MATα eco1W216G::hphMX ade2-1 bar1Δ can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 | 33 | |
| JSC52 | MATa hmrΔ::RS-HMR-tT(AGU)C-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 eco1W216G::hphMX bar1Δ can1-100 his3-11,15 trp1-1 ura3-1 | cross of RDY215 and SG179.4 | This study |
| JSC81 | MATa hmr-git1Δ::RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 eco1W216G::hphMX bar1Δ can1-100 his3-11,15 trp1-1 ura3-1 [pJSC2 (CEN/ARS/TRP1/256xlacop)] | cross of RDY215 and SG179.4 | This study |
| JSC96 | MATa hmrΔ::RS-loxP-URA3r-loxP-GIT1-TRP1-256xlacop-RS Δhml::(loxP-kanMX-loxP)2 ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 scc1-73 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3-1 | RDY213 and PCR template strain RRY5 (H. Madhani) | This study |
| JSC97 | MATa hmrΔ::RS-loxP-URA3r-loxP-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3-1 | RDY215 and PCR template strain RRY5 (H. Madhani) | This study |
| JSC157 | MATa hmrΔ::RS-loxP-URA3r-loxP-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3Δ::SpHIS5MX | JSC97 | This study |
| JSC164 | MATα hmrΔ::RS-loxP-URA3r-loxP-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 ycs4-2::natMX bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3-1 | ycs4-2 from PCR template pSO6 (B. Lavoie) | This study |
| JSC183 | MATa hmrΔ::RS-URA3f-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3-1 | RDY215 and PCR template pER7 (A. Bresson) | This study |
| JSC196 | MATa hmrΔ::RS-6lexopssEB-a2a1–256lacop-TRP1-Δhmri-RS ade2-1::HIS3p-lacGFP::ADE2 (LEU2::GAL1p-R)2::leu2-3,112 kanMX::MET3p-CDC20 can1-100 his3-11,15 trp1-1 ura3-1 | CSW19 (56) | This study |
| JSC208 | MATa hmrΔ::RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 smc3-42 can1-100 his3-11,15 trp1-1 ura3-1 [pJSC2 (CEN/ARS/TRP1/256xlacop)] | CSW48 (56) | This study |
| JSC209 | MATa hmr-git1Δ::RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3-1 [pJSC2 (CEN/ARS/TRP1/256xlacop)] | RDY215 | This study |
| JSC230 | MATa hmrΔ::RS-loxP-URA3r-loxP-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 MCD1-TAP::natMX kanMX::MET3p-CDC20 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3Δ::SpHIS5MX | JSC157 | This study |
| MSB7 | MATa hmrΔ::RS-URA3f-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 kanMX::MET3p-CDC20 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3-1 | cross of JSC183 and JSC196 | This study |
| MSB13 | MATa hmrΔ::RS-loxP-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 kanMX::MET3p-CDC20 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3Δ::SpHIS5 | JSC97 | This study |
| MSB14 | MATa hmrΔ::RS-URA3f-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 kanMX::MET3p-CDC20 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3Δ::SpHIS5MX | MSB7 | This study |
| MSB19 | MATa hmrΔ::RS-URA3f-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 MCD1-TAP::natMX kanMX::MET3p-CDC20 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3Δ::SpHIS5MX | MSB14 | This study |
| MSB20 | MATa hmrΔ::RS-loxP-URA3r-loxP-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 kanMX::MET3p-CDC20 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3Δ::SpHIS5MX | JSC157 | This study |
| MSB30 | MATa hmrΔ::RS-URA3f-loxP-SpHIS5MXf-loxP-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 kanMX::MET3p-CDC20 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3-1 | MSB7 | This study |
| MSB31 | MATa hmrΔ::RS-URA3f-loxP-SpHIS5MXr-loxP-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 kanMX::MET3p-CDC20 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3-1 | MSB7 | This study |
| MSB35 | MATa hmrΔ::RS-URA3f-loxP-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 kanMX::MET3p-CDC20 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3-1 | MSB31 | This study |
| MSB37 | MATa MCD1-TAP::natMX kanMX::MET3p-CDC20 ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 | PJ1 | This study |
| MSB105 | MATa hmrΔ::RS-URA3f-loxP-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 kanMX::MET3p-CDC20 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3Δ::SpHIS5MX | MSB35 | This study |
| MSB110 | MATa hmrΔ::RS-URA3f-loxP-SpHIS5MXf-loxP-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 kanMX::MET3p-CDC20 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3Δ::hphMX | MSB30 | This study |
| MSB112 | MATa hmrΔ::RS-URA3f-loxP-SpHIS5MXr-loxP-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 kanMX::MET3p-CDC20 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3Δ::hphMX | MSB31 | This study |
| MSB123 | MATα SCC2-AID-9myc::kanMX MCD1-TAP::natMX kanMX::MET3p-CDC20 ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 | cross of MSB37 and YFC89 | This study |
| MSB128 | MATα SCC2-AID-9myc::kanMX trp1-1::ADH1p-OsTIR1::TRP1 MCD1-TAP::natMX kanMX::MET3p-CDC20 ade2-1 can1-100 his3-11,15 leu2-3,112 | MSB123 | This study |
| MSB147 | MATa hmrΔ::RS-loxP-URA3r-loxP-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 smc3-42 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3-1 | smc3-42 crossed in from strain K5824 | This study |
| MSB153 | MATa MCD1-TAP::natMX kanMX::MET3p-CDC20 ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3e-Δ(uf-da:dt)-loxP [pXR-klURA3-HIS3] | MSB37 | This study |
| MSB155 | MATα lys2Δ::RS-lys2-dA:dT-loxP-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 can1-100 his3-11,15 trp1-1 | CSW91 | This study |
| MSB156 | MATa hmrΔ::RS-URA3f-Δ(uf-da:dt)-loxP-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 MCD1-TAP::natMX kanMX::MET3p-CDC20 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3Δ::hphMX [pXR-klURA3-HIS3] | MSB19 | This study |
| MSB157 | MATa hmrΔ::RS-loxP-URA3r-loxP-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 eco1W216G::hphMX bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3-1 | cross of JSC97 and SG179.4 | This study |
| HJ4 | MATa hmrΔ::RS-ura3f-fulltrunc-loxP-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 kanMX::MET3p-CDC20 bar1Δ::hisG can1-100 his3-11,15 trp1-1 | MSB7 | This study |
| HJ5 | MATa hmrΔ::RS-ura3f-orftrunc-loxP-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 kanMX::MET3p-CDC20 bar1Δ::hisG can1-100 his3-11,15 trp1-1 | MSB7 | This study |
| HJ6 | MATa hmrΔ::RS-ura3f-tatatrunc-loxP-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 kanMX::MET3p-CDC20 bar1Δ::hisG can1-100 his3-11,15 trp1-1 | MSB7 | This study |
| HJ7 | MATa hmrΔ::RS-ura3f-uastrunc-loxP-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 kanMX::MET3p-CDC20 bar1Δ::hisG can1-100 his3-11,15 trp1-1 | MSB7 | This study |
| HJ12 | MATa hmrΔ::RS-ura3f-fulltrunc-loxP-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 kanMX::MET3p-CDC20 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3-1 | MSB7 | This study |
| HJ19 | MATa hmrΔ::RS-ura3f-da:dttrunc-loxP-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 kanMX::MET3p-CDC20 bar1Δ::hisG can1-100 his3-11,15 trp1-1 | MSB7 | This study |
| HJ22 | MATα lys2Δ::RS-lys2-URA3p-loxP-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 can1-100 his3-11,15 trp1-1 | CSW91 | This study |
| HJ23 | MATα lys2Δ::RS-lys2-UF-dA:dT-loxP-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 can1-100 his3-11,15 trp1-1 | CSW91 | This study |
| HJ24 | MATa hmrΔ::RS-ura3f-Δuf-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 kanMX::MET3p-CDC20 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3-1 [pXR-URA3-HIS3] | HJ12 | This study |
| HJ25 | MATa hmrΔ::RS-ura3f-nrg1mut-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 kanMX::MET3p-CDC20 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3-1 [pXR-URA3-HIS3] | HJ12 | This study |
| HJ6388 | MATa hmrΔ::RS-ura3f-Δda:dt-GIT1-TRP1-256xlacop-RS ade2-1::HIS3p-GFPlacI::ADE2 leu2-3,112::(GAL1p-RecR::LEU2)2 bar1Δ::hisG can1-100 his3-11,15 trp1-1 ura3-1 [pXR-URA3-HIS3] | RDY215 and PCR template pER7-Δpoly(dA-dT)1 (A. Bresson) | This study |
| YFC89 | MATα SCC2-AID-9myc:kanMX ura3-1::ADH1p-OsTIR1::URA3 MCD1-TAP::natMX ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 | W303-1A | This study |
All strains were derived from W303-1A.