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Perfusion-induced FTIR difference spectra of LacY G46W/G262W in the presence of NPG in both perfusion solutions. The sample equilibrated at pH 7.0 was subtracted from the sample equilibrated at 9.0 (A), 10.0 (B), 10.5 (C), 10.9 (D), and 11.5 (E), respectively.
