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Position of Glu325 in C-terminal six-helix bundle of WT LacY (Protein Data Bank ID code 2V8N). LacY is presented as rainbow-colored backbone (from blue to red for helices 1–12) with hydrophilic cavity open to cytoplasmic side. Side chain of Glu325 located in helix X is shown as spheres. The area around Glu325 is enlarged with hydrophobic environment displayed as a space-filled cartoon (cyan).
