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. 2017 Jan 30;114(7):1726–1731. doi: 10.1073/pnas.1613375114

Table S3.

List of primers used in the study

Amplicon and primer Sequence (5′–3′) Annealing temperature (°C)
Amplicon 1
 ITS-Poa-f AAGGATCATTGTCGTGACC 51
 ITS4 TCCTCCGCTTATTGATATGC
Amplicon 2
 ITS-Poa-f AAGGATCATTGTCGTGACC 51
 ITS-Pan-bac ACACTGCACCGAGAACAAC
Amplicon 3
 Pas_141F ATATTGCCTTGCGAGGGTGA 55
 Pas_435R TGCACCATGGCACGAGAG
 Pan1_162F GTTCGGCCTGTCGCGCAT 55
 Pan1_486R ACATGCAGGTGCCCGACA
 Pan2_65F AAGGCCCCCAGTCAACATTT 60
 Pan2_484R CGTGCAAGTACCTAACACGG
 Euc_140F TATTGACTTGCTCGGCGGAG 60
 Euc_481R TGCTGTGCCCGATACGATTC
 Set_31F CGTGTCATCCATGCCGCAT 55
 Set_202R AGATAGCATCGTTACACGAGGT
 Aru_49F GGGCTTCGGTCCGGTTTA 54
 Aru_168R GCCGACCGCTCCACCGT

ITS-Poa-f is from ref. 34. ITS4 is from ref. 55. ITS-Pan-bac is from ref. 18. Genus-specific primers are from this study.