Table S3.
Amplicon and primer | Sequence (5′–3′) | Annealing temperature (°C) |
Amplicon 1 | ||
ITS-Poa-f | AAGGATCATTGTCGTGACC | 51 |
ITS4 | TCCTCCGCTTATTGATATGC | |
Amplicon 2 | ||
ITS-Poa-f | AAGGATCATTGTCGTGACC | 51 |
ITS-Pan-bac | ACACTGCACCGAGAACAAC | |
Amplicon 3 | ||
Pas_141F | ATATTGCCTTGCGAGGGTGA | 55 |
Pas_435R | TGCACCATGGCACGAGAG | |
Pan1_162F | GTTCGGCCTGTCGCGCAT | 55 |
Pan1_486R | ACATGCAGGTGCCCGACA | |
Pan2_65F | AAGGCCCCCAGTCAACATTT | 60 |
Pan2_484R | CGTGCAAGTACCTAACACGG | |
Euc_140F | TATTGACTTGCTCGGCGGAG | 60 |
Euc_481R | TGCTGTGCCCGATACGATTC | |
Set_31F | CGTGTCATCCATGCCGCAT | 55 |
Set_202R | AGATAGCATCGTTACACGAGGT | |
Aru_49F | GGGCTTCGGTCCGGTTTA | 54 |
Aru_168R | GCCGACCGCTCCACCGT |