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. 2017 Feb 2;114(7):E1158–E1167. doi: 10.1073/pnas.1614364114

Fig. 2.

Fig. 2.

DNA damage leads to transcriptional up-regulation of autophagy-related genes in a Rad53-dependent manner. (A) Transcript abundance of autophagy genes in sml1Δ and rad53Δ sml1Δ mutants were assayed 0, 1, and 3 h after MMS treatment. *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t test. (B) Overexpression (OE) of Rph1 prevents transcriptional up-regulation of autophagy genes. (C) Overexpression of Rph1 inhibits Atg8 induction and lipidation after MMS-induced DNA damage. Quantification of the blot is presented to the Right. *P < 0.05, **P < 0.01. (D) Rph1 is phosphorylated by Rad53 after MMS. Strains of the indicated genotype were treated with 0.04% MMS and analyzed for Rph1 phosphorylation by Western blot. (Left) A Phos-tag gel. (Right) SDS/PAGE gel without phos-tag. (E) The rph1Δ cells have higher autophagy after DNA damage. Quantification of the blot is presented to the Right. **P < 0.05, ***P < 0.01. Error bars reflect SD from 3 independent experiments.