Endosomal MYO6 binding partners are reorganized by MYO6+. (A) HeLa cells were transfected with GFP-MYO6 or GFP-MYO6+, fixed, stained for GIPC, and imaged by confocal microscopy. (B) HeLa cells were transfected with GFP-MYO6 or GFP-MYO6+, fixed, stained for APPL1, and imaged by confocal microscopy. (Scale bars, 10 µm.) (C) Pearson’s correlation coefficients were calculated for cells expressing either MYO6 or MYO6+ and stained with anti-GIPC or anti-APPL1 as indicated (more than 30 cells from three independent experiments).