Fig. S7.

Model of filopodia formation induced by MYO6+. (i) MYO6 monomers (dark blue) bind to APPL1 endosomes (light green) via the cargo adaptor GIPC (light blue), forming either tethered monomers or dimers. These motors link APPL1 to the peripheral actin network (red) near the cell boundary (black). (ii) The movement of MYO6+ toward the plus ends of actin filaments leads to cross-linking, bending, and condensation of actin and accumulation of APPL1 endosomes near the cell membrane. (iii) The continued action of MYO6+ leads to the extension of bundles of actin, which stabilize inside the membrane sheath. APPL1 endosomes are excluded from the dense core of the filopodium, leading to detachment of MYO6+ complexes containing a multitude of cargoes, which accumulate at the tips.