Table S2.
Probabilities of distinct configurations of GltPh trimers
| Condition | p(U) | p(D) | p(UUU) | p(UUD) | p(UDD) | p(DDD) |
| Apo (no substrates) | 0.79 | 0.21 | 0.49 | 0.39 | 0.11 | 0.01 |
| Transport (Na+ + Asp) | 0.84 | 0.16 | 0.60 | 0.34 | 0.05 | 0.004 |
The state probabilities of individual elevator domains, p(U) (column 1) and p(D) (column 2), were derived from the experimental dwell time τ(up) and τ(down) according to Eqs. S4 and S5, respectively. Probabilities of transport domain configurations (columns 3, 4, 5, and 6) within a full noncooperative trimer were calculated using Eq. S3.