Table 1.
Compiled quantitation data from in vitro, cell culture, and in vivo experiments on nsP3 WT and mutants of CHIKV 181/25 strain
| CHIKV strain | ADP-ribose KD, μM | Hydrolase activity, % | Virus progeny | Sequencing | Replication (NSC-34), % | Mortality in mice, % | Mean days of death |
| WT | 22.9 ± 3.7 | 100 | Yes | 100 | 100 | 2.5 | |
| Category 1 | |||||||
| D10A | NDB | 16.1 ± 2.3 | No | Reverted to WT | N/A | N/A | N/A |
| G32A | 21.0 ± 3.7 | 75.3 ± 9.3 | Yes | 7 | 58 | 6.9 | |
| G32S | 42.9 ± 17.7 | 48.0 ± 16.0 | Yes | 4 | 4 | Undefined | |
| G32E | NDB | 3.8 ± 9.1 | No | Reverted to WT | N/A | N/A | N/A |
| T111A | 71.4 ± 11.5 | 64.1 ± 4.0 | Yes | <1 | 44 | 5.3 | |
| G112E | NDB | 3.7 ± 2.5 | No | Reverted to WT | N/A | N/A | N/A |
| R144A | 64.9 ± 14.9 | 96.2 ± 2.3 | No | Reverted to WT | N/A | N/A | N/A |
| Category 2 | |||||||
| Y114A | 4.84 ± 0.69 | 40.6 ± 4.2 | Yes | 16 (Delayed) | 75 | 5.3 | |
| G32E V113R Y114N | 6.46 ± 1.25 | 15.0 ± 7.2 | No | Reverted to either G32A V113R Y114N or V113R Y114N | N/A | N/A | N/A |
Hydrolase activity is calculated as a percentage of nsP3 WT activity after subtraction of buffer control. Replication rate is defined as the number of plaques in a mutant divided by the number of plaques in WT. CHIKV 181/25E2 I12T R82G and mutant derivatives were used in mouse experiments. N/A, not available; NDB, no detectable binding.