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. 2017 Jan 30;114(7):1702–1707. doi: 10.1073/pnas.1618606114

Fig. 5.

Fig. 5.

DSCAM promotes RGC axon outgrowth in situ. (A) Schematic diagram of the culture approach. Ventrotemporal retinal explants, containing predominately ipsilaterally projecting RGCs, and brain slices at the level of the optic tract were prepared from E16.5 Dscamdel17 mutant and WT littermates. Retinal explants were cultured on top of the brain slices in different genetic combinations. (B) Bright-field and fluorescent (DiI) images of an E16.5 WT retinal explant cultured on a WT brain slice. (C) Manual tracing of RGC axons from Dscamdel17 WT (gray) or mutant (blue) retinal explants cultured on WT (gray) or mutant (blue) brain slices. (D) Mean ± SEM RGC axon outgrowth from E16.5 Dscamdel17 WT and mutant retinal explants cultured on brain slices from WT and mutant embryos. Numbers on the bars indicate the numbers analyzed. Results are from five independent experiments analyzed blinded to genotype. *P < 0.05; **P < 0.01; ***P < 0.001.