Fig. 3.
Validation that a mutation in Gatm causes the mrbig phenotype. (A) Weight loss analysis of the Gatmmb/c mice in response to 1.5% DSS. Gatm+/+ (n = 3), Gatmmb/+ (n = 4), Gatmc/+ (n = 5), and Gatmmb/c (n = 5) mice were analyzed on day 8 of treatment. ***P < 0.001. (B) Colons of Gatmmb/c mice exposed to DSS on day 8 of treatment exhibited shortening (n = 5). Gatm+/+ (n = 3) mice were also analyzed. **P < 0.01. (C) Stool scores in Gatmmb/c mice 7 d after administration of 1.5% DSS. Gatm+/+ (n = 3) and Gatmmb/c (n = 5) mice were analyzed. **P < 0.01. (D) Relative mRNA expression levels of inflammatory cytokines Cxcl2, Il6, and Tnf in the distal colon of Gatm+/+ (n = 3) and Gatmc/c (n = 3) mice after 6 d of 1.5% DSS. Experiment was repeated three times; *P < 0.05. (A–D) P values were calculated by Student’s t test; bars indicate mean ± SD.