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. 2017 Jan 30;114(7):E1273–E1281. doi: 10.1073/pnas.1621400114

Fig. S4.

Fig. S4.

Mitochondrial mass and functioning in Gatmc/c colonocytes. (A) Colonocytes from untreated and 3 d DSS-treated Gatmc/c (blue) vs. Gatm+/+ littermates (red) were harvested and stained with MitoTracker Green. Data are representative of three independent experiments. (B) Mitochondrial respiration of colonic crypts from mice of the indicated genotypes treated for 3 d with 1.5% DSS was tested using Seahorse Flux. Oligomycin, carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP), and a mix of rotenone and antimycin A were serially injected to measure ATP-linked respiration, maximal respiration, and nonmitochondrial respiration, respectively. Data are representative of three independent experiments.