Fig. 4.
EBI3-deficient mice show decreased MCMV replication in the salivary glands and oral lavage. (A) Expansion of peripheral KLRG1hi Ly49H+ NK cells from WT or Ebi3−/− B6 mice was measured by flow cytometry at days 0, 7, 14, 21, and 28 p.i. n = 6 for each mouse strain and time point from two independent experiments (mean ± SD). (B) CD69 expression and (C) IFNγ production was measured by flow cytometry in splenic Ly49H+ and Ly49H– NK cells from WT or Ebi3−/− B6 mice at day 1.5 post-MCMV infection. n = 4 for each mouse strain from two independent experiments. Data show mean ± SD. MCMV titer in WT or Ebi3−/− B6 mice was determined by real-time PCR in (D) liver and (E) spleen at day 4 p.i. in (F) peripheral blood at days 3, 7, and 14 p.i., in (G) saliva (by oral lavage) at day 14 and day 28 p.i., and (H) in salivary glands at day 28 p.i. n = 4 or 6 for each mouse strain and time point from two independent experiments. Statistical analysis was performed by two-tailed unpaired Student’s t test (*P < 0.05 and ***P ≤ 0.0003).