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. 2017 Jan 30;114(7):E1215–E1223. doi: 10.1073/pnas.1613609114

Table S4.

Plasmids used in this study

Plasmid Description Source
pWQ573 Plasmid vector with l-arabinose-inducible promoter; Cmr 52
pWQ719 pWQ573 derivative encoding WbbB-FLAG from Raoultella terrigena; Cmr This study
pWQ673 pWQ552 derivative encoding WbbL-Wzm-Wzt from R. terrigena; Apr This study
pWQ672 pWQ552 derivative encoding WbbL-Wzm-Wzt-WbbB from R. terrigena; Apr This study
pWQ698 pWQ573 derivative encoding His6-WbbB504-1106 ; Cmr This study
pWQ701 pWQ573 derivative encoding His6-WbbB402-1106; Cmr This study
pWQ702 pWQ573 derivative encoding His6-WbbB1-401; Cmr This study
pWQ676 pWQ552 derivative encoding WbbL-Wzm from R. terrigena, Apr This study
pWQ677 pWQ552 derivative encoding WbbL-Wzm-WbbB from R. terrigena, Apr This Study
pET28a(+) Plasmid vector with IPTG-inducible promoter; Kmr Novagen
pET30a(+) Plasmid vector with IPTGinducible promoter; Kmr Novagen
pRmlB2 pET30a(+) derivative encoding His6-RmlB from Salmonella enterica; Kmr 47
pRmlC3 pET30a(+) derivative encoding His6-RmlC from S. enterica; Kmr 47
pRmlD2 pET30a(+) derivative encoding His6-RmlD from S. enterica; Kmr 47
pWQ867 pET28a(+) derivative encoding His6-WbbB540-1106; Kmr This study
pWQ868 pWQ719 derivative encoding WbbB-FLAG with native coiled-coil domain (6, 6) and introduced NotI and KpnI restriction sites; Cmr This study
pWQ869 pWQ868 derivative encoding WbbB-FLAG with altered coiled-coil domain (2, 6); Cmr This study
pWQ870 pWQ868 derivative encoding WbbB-FLAG with altered coiled-coil domain (6, 2); Cmr This study
pWQ871 pWQ868 derivative encoding WbbB-FLAG with altered coiled-coil domain (10, 6); Cmr This study
pWQ872 pWQ868 derivative encoding WbbB-FLAG with altered coiled-coil domain (6, 10); Cmr This study
pWQ873 pWQ868 derivative encoding WbbB-FLAG with altered coiled-coil domain (2, 2); Cmr This study
pWQ874 pWQ719 derivative encoding WbbB-FLAG with altered coiled-coil domain (12,0); Cmr This study
pWQ875 pWQ719 derivative encoding WbbB-FLAG (0,0)with the region encompassing the entire coiled-coil domain (residues 402–539) removed; Cmr This study
pWQ677 pWQ552 derivative encoding WbbL-Wzm-WbbB from R. terrigena; Apr This study
pWQ876 pWQ719 derivative encoding WbbB-FLAG with altered coiled-coil domain (10, 10); Cmr This study
pWQ877 pET28a(+) derivative encoding His6-WbbBΔR738AR739AK740A; Kmr This study
pWQ878 pET28a(+) derivative encoding His6-WbbB576-871; Kmr This study