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. 2017 Jan 30;114(7):E1224–E1233. doi: 10.1073/pnas.1614893114

Fig. 3.

Fig. 3.

Hap1 sequesters Dcaf7 in the cytoplasm. (A) Immunostaining of Dcaf7 when transfected alone or cotransfected with Hap1A or Hap1B into HEK293 cells. (Scale bar: 8 µm.) (B) Subcellular fractionation of HEK293 cells transfected with Hap1B or a control vector. Western blotting (Upper) and quantification (Lower) of total (T), cytoplasmic (C), and nuclear (N) fractions indicate a significant enrichment of Dcaf7 in the cytoplasm when Hap1B is present. Ratios were normalized to control level. n = 4 each. (C) Subcellular fractionation of P2 WT and Hap1-KO mouse brains. Western blotting (Upper) and quantification (Lower) show a decreased cytoplasmic/nuclear ratio of Dcaf7 in Hap1-KO brains. n = 3 each. (D) Coimmunostaining of Dcaf7 and Hap1 in postnatal day 2 WT and Hap1-KO mouse hypothalamus displays differential distributions of Dcaf7. (Scale bar: 10 µm.) *P < 0.05.