Fig. 6.

Overexpression of DYRK1A in mouse hypothalamus reduces the Hap1–dcaf7 interaction and leads to reduced body weight gain. (A) IP of Hap1 in the DYRK1A-overexpressing DS mouse model. Ts65Dn hypothalamus reveals a decreased association of Hap1 and Dcaf7. (B) Immunostaining of Myc in postnatal day 20 WT mouse hypothalamus that had been injected with AAV-DYRK1A-Myc or AAV-control at postnatal day 2. Enlarged images of the boxes are shown below. (Scale bars: 15 µm.) (C) Body weight recording indicates that DYRK1A overexpression in the hypothalamus reduced the postnatal weight gain of WT mice. n = 11–20. *P < 0.05; **P < 0.01.