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Hap1 sequesters Dcaf7 in the cytoplasm. (A) Transfection of Hap1A or Hap1B, but not Ahi1, in HEK293 cells redirects a great portion of endogenous Dcaf7 to the cytoplasm. Arrows indicate cells with redistributed Dcaf7. (B) Cotransfection of Dcaf7-HA and Hap1A in PC12 cells shows punctate cytoplasmic staining for both, whereas transfecting Dcaf7-HA alone gives diffuse staining of Dcaf7 with more nuclear signal. (Scale bars: 10 µm.)
