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. 2017 Jan 30;114(7):1631–1636. doi: 10.1073/pnas.1619667114

Fig. S2.

Fig. S2.

(A) IL-37 has no effect on BMP-2 levels in diseased human AVICs in the absence of stimulation. Diseased AVICs were treated with different concentrations of recombinant IL-37 (0.01–10.0 ng/mL) for 3 d. Levels of BMP-2 protein were assessed with immunoblotting. A representative immunoblot of three separate experiments show that recombinant IL-37 has no effect on cellular BMP-2 levels in the absence of proinflammatory stimulation. (B) Recombinant IL-37 reduces calcium deposit formation in AVICs of diseased aortic valves following in vitro stimulation with proinflammatory stimuli. Spectrophotometric data (n = 5 separate experiments using distinct cell isolates) show that recombinant IL-37 reduces calcium deposit formation in diseased cells after 21 d of stimulation in a conditioning medium. *P < 0.05 vs. corresponding control; ‡P < 0.05 vs. stimulant alone.