Fig. S4.

The inhibitory activity of 15 in agonist-induced β-arrestin endocytosis. After pretreatment with 15 at different concentrations for 20 min, the level of β-arrestin endocytosis (Endo), upon stimulation of (A) β₂V₂R, (B) V₂R, and (C) AT₁R with respective agonists in a dose-dependent manner, was monitored. Values were expressed as percentages of the maximal level of the activity induced by the agonist of each receptor in the vehicle (0.5% DMSO) control. Points on curves represent mean ± SEM obtained from at least three independent experiments done in duplicate. AngII, angiotensin II; AVP, arginine vasopressin; ISO, isoproterenol.