Fig. 1.

Characterization of the cell lines with the FL-HR reporter on the inactive and active X chromosome. (A) Luciferase activity of cells with the reporter on the inactive X (Xi-8) with varying concentrations of 5-AZA, and the reporter on the active X (Xa-3). All measurements were performed in triplicate; error bars indicate SD. LU, light unit; NT, vehicle treated. (B) Western blot showing the presence or absence of the WT copy of MeCP2 in Xi-8 and Xa-3 cells. Brain lysates from 4-wk-old MeCP2/Y and MeCP2-FL-HR/y animals were included as controls. (C) Hygromycin B resistance of cells with the reporter on the active (Xa-3) and inactive (Xi-8) X chromosome. Cells were subjected to hygromycin B at a similar dose and duration (20 µg/mL for 6 d) as was used in the screen, and growth was normalized to untreated controls. Error bars indicate SD; n = 3, P < 0.001. (D) Screen design.