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. 2017 Jan 31;114(7):1619–1624. doi: 10.1073/pnas.1621356114

Fig. 4.

Fig. 4.

Effect of TGF-β signaling on XIST in Xi-8 cells and in mice. (A) TGF-β down-regulates XIST, whereas inducing three known TGF-β targets in Xi-8 cells. Cells were treated with TGF-β1 (5 ng/mL) for the indicated times, and levels of XIST and mRNA for PAI-1, SnoN, and Smad7 were measured by RT-qPCR. Error bars indicate SD. All measurements were carried out in quadruplicate. *P < 0.05, **P < 0.01, ***P < 0.001. (B) Albumin-Cre–mediated excision of Tgfbr2 in female mouse livers induces XIST and represses Smad7. Mice with liver-specific knockout of Tgfbr2 (Tgfbr2LKO) were generated by albumin-Cre–mediated deletion of the floxed Tgfbr2 gene (Tgfbr2 co/co). Cre-Tgfbr2 (co/co) littermate controls have an intact Tbfbr2. XIST RNA, Smad7, Kdmc5, and Ddx3x mRNA were measured by RT-qPCR. The levels of XIST, Smad7, Kdmc5, and Ddx3x mRNA in Cre-Tgfbr2 (co/co) animals were set to 1. *P < 0.05, **P < 0.01. Error bars indicate SD.